Petaka G3 LOT in an open environment, with 21% oxygen, works like a hypoxia chamber with an atmosphere of 2% oxygen; Petaka G3 will dissipate the oxygen dissolved in the media like a flask or dish will do inside an incubator with 2% oxygen.
Petaka G3 has a proportional valve system (PVS) controlling the in/out gas diffusion, and a diffusion restriction system (DRS).
If the partial pressure of a gas in the media is lower than in the atmosphere, the PVS allows the diffusion of gas in the media until both partial pressures are balanced, and vice versa.
The diffusion restriction system (DRS) imposes a particular and predetermined intake diffusion rate (PDR) in each Petaka G3 type. The DRS quenches heavily the gas diffusion in both directions, allowing the progressive gas depletion or slow repletion.
In effect, if the cell population consumes more oxygen than the PDR, the cells progressively deplete the dissolved oxygen until the minimal biologic oxygen consumption rate (mbOCR) of the cell population equals the PDR, then the inner and external oxygen in and out diffusion will be intermittently balance and the media will exhibit a flat pulsing level of dissolved oxygen. That level will be characteristic of the cell type and the functional conditions of the cells.
