The Petaka G3 LOT under atmospheric conditions with 21% oxygen, provides a culture environment like a hypoxia chamber with an atmosphere of ~2% oxygen. The Petaka G3 will automatically dissipate the oxygen dissolved in the media just like a flask or dish does inside an incubator with ~2% oxygen.
Petaka G3 has a proportional valve system (PVS) controlling the in/out gas diffusion, and a diffusion restriction system (DRS).
If the partial pressure of a gas in the media is lower than in the atmosphere, the PVS allows the diffusion of gas in the media until both partial pressures are balanced, and vice versa.
The diffusion restriction system (DRS) imposes a predetermined intake diffusion rate (PDR) in each Petaka G3 device. The DRS
heavily restricts the gas diffusion in both directions, allowing controlled progressive gas depletion or slow replenishment.
In effect, as the cell population consumes more oxygen than the PDR, the cells progressively deplete the internal dissolved oxygen until the minimal biologic oxygen consumption rate (mbOCR) of the cell population equals the PDR, then the inner and external oxygen (in and out) diffusion will be intermittently balance and the media will exhibit a flat pulsing level of dissolved oxygen. The minimal oxygen level in a culture in Petaka G3 will vary among various cell types since the physiologic oxygen level of each cell type in the body is different. The Petaka G3 automatically provides this unique tissue-specific oxygen level for each cell type cultured in the device.
